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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in lots of tumors, which leads to chromosome instability, and so may add to tumorigenesis. This protein was located to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial job in endorsing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already described. Two more splice variants had been claimed but specific nucleotide sequence info is not really nevertheless available.
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Blocking peptides are peptides that bind especially towards the focus on antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This tutorial outlines further more probable optimization of cellular and immunoassay parameters to be sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Target Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose customers are characterised by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation styles which contribute to your temporal coordination of each and every mitotic event. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells development as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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